| Date / Time | Teams |
 |  16/09/2025 15:00 | Apeldoorn - Brickfield Rangers F.C. |
| 16/09/2025 15:00 | Sittard - LFC Slovan Alkmaar |
| 16/09/2025 15:00 | Feyenoord Rotterdam™ - N.E.C. Nijmegen |
| 16/09/2025 15:00 | Cisowa Tokio BOYS - F Rotterdam |
| 16/09/2025 15:00 | Leerdam - PSV EINDHOVEN |
| 16/09/2025 15:00 | Michu KS - Midwolda |
| 16/09/2025 15:00 | Utrecht - VITESE ARNHEIM |
| 16/09/2025 15:00 | Hooghalen - FC Hattfut Den Bosch |
| 16/09/2025 15:00 | Excelsior - FC Utrecht |
| 16/09/2025 15:01 | FC Breda - AFC Ajax Amsterdam A |
| 16/09/2025 15:01 | Thorn - Uithuizen |
| 16/09/2025 15:01 | Ajax Amsterdam™ - Arnemuiden |
| 16/09/2025 15:01 | Vlissingen - Kakucs A |
| 16/09/2025 15:01 | Drzewica - PSV Eindhoven A |
| 16/09/2025 15:01 | Helmond - FC Breda |
| 16/09/2025 15:01 | Groningen - CF.Vitesse Arnhem™ |
